Investigating the Bioactive Compounds in Leaves’ Extracts of Gossypium barbadense Species using High-Performance Liquid Chromatography
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Abstract
Gossypium barbadense leaves have been used traditionally in the treatment of several ailments such as asthma, pyrexia and yellow fever, with no much scientific scrutiny of its acclaimed efficacy. This study investigated the bioactive components of hybrid and traditional Gossypium barbadense leaves using high-performance liquid chromatography (HPLC). Extraction of samples were carried out by maceration using 95% ethanol. Bioactive compounds were determined using agilent technology; high-performance liquid chromatography. HPLC analysis revealed presence of bioactive compounds in the hybrid leaf, such as: eugenol 1.522 mg/g. camphor 0.128 mg/g, alpha-tumerone 0.095 mg/g, epicatechin 0.118 mg/g, alpha-pinene 0.105 mg/g. bioactive compounds in the tradition leaf include: ellagic acid 1.531 mg/g, Zeaxanthine 0.927 mg/g, P-coumaric acid 0.409 mg/g, catechin 0.279 mg/g. This study revealed that leaves of hybrid and traditional Gossypium barbadense have different bioactive compounds. Quercetin and epicatechin were bioactive compound they have in common and the traditional plant is richer in bioactive compounds. Difference in the compositions of the plant species is influenced by genetic modification of the hybrid specie. Hybrid specie is much useful for production of textile materials, traditional species with more bioactive compounds is considered for treatment of diseases.
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